Description: This product includes 60 ul of 1000 x PSA dye for more than 200 assays with the assay volume of 0.3 ml using 96-well plates or cuvettes.

Description: The ARH3 Fluorogenic Assay Kit is a high-throughput, homogeneous 96-well assay designed to measure the hydrolase activity of ARH3 (ADP-ribosylhydrolase) for screening and profiling applications, using a simple and straightforward fluorogenic assay. The ARH3 Fluorogenic Assay Kit contains enough purified recombinant ARH3 enzyme, substrate, and assay buffer for 100 enzyme reactions. The kit includes the ARH3 inhibitor ADP-HPD as an ARH3 activity control.Figure 1: Illustration of the assay principle.ARH3 is incubated with a fluorogenic ADP-ribose substrate in which the fluorophore is quenched by the presence of the ribose. ARH3-mediated hydrolysis of the substrate between the ribose and the fluorochrome releases fluorescence that can be detected at λ=502 nm (excitation at λ=385 nm) . Fluorescence intensity is directly proportional to ARH3 hydrolase activityNeed us to run inhibitor screens or profile your compounds against ARH3? Check out ourDNA Replication & Repair Screening Services.

Description: The GPX4 Fluorogenic Assay Kit is a fluorogenic assay designed to measure GPX4 (glutathione peroxidase 4) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant GPX4, Glutathione, Cumene Hydroperoxide, NADPH, Glutathione Reductase, and GPX4 Assay Buffer for 100 reactions.Figure 1: Mechanism of detection used in the GXP4 Assay Kit.GXP4 activity is measured indirectly. GXP4 converts reduced glutathione (GSH) to oxidized glutathione (GSSG) by reducing hydroperoxide (ROOH) . GSSG is then converted back to its reduced form by glutathione reductase (GR) in the presence of NAPDH, which is converted into NADP+. NADPH is intrinsically fluorescent, a property lost upon oxidation to NADP+. The decrease in fluorescence (exc λ=340 nm; em λ=450 nm) observed is proportional to the amount of GSSH available for conversion by GR, and to GXP4 activity.

Description: The Fluorogenic HDAC1 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified_x000D_HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC1. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC4 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 4 (HDAC4) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC4 activity measurements. In addition, the kit includes purified_x000D_HDAC4 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC4 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC4 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC4. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D__x000D_

Description: The Fluorogenic HDAC5 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 5 (HDAC5) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC5 activity measurements. In addition, the kit includes purified_x000D_HDAC5 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC5 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC5 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC5. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC7 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 7 (HDAC7) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC7 activity measurements. In addition, the kit includes purified_x000D_HDAC7 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC7 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC7 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC7. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC 8 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) 8 activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC activity measurements. In addition, the kit includes purified_x000D_HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative_x000D_control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate_x000D_and developer combination. This assay method eliminates dealing with the radioactivity,_x000D_extraction, and chromatography aspects of traditional assays. Using this kit, only twoa_x000D_simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First,_x000D_the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. The_x000D_deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence_x000D_reader.

Description: The Fluorogenic HDAC9 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 9 (HDAC9) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC9 activity measurements. In addition, the kit includes purified_x000D_HDAC9 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC9 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC9 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC9. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC3 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 3 (HDAC3) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified_x000D_HDAC3 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC3 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader.

Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_ HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_ substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

Description: The Fluorogenic MMP11 Assay Kit is designed to measure MMP11 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

Description: The Fluorogenic MALT1 Assay Kit is designed to measure MALT1 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Description: The Fluorogenic MMP10 Assay Kit is designed to measure MMP10 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

Description: The Fluorogenic MMP13 Assay Kit is designed to measure MMP13 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.