Developmental data for several human mitochondrial DNA (mtDNA) long amplification targets
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Developmental data for several human mitochondrial DNA (mtDNA) long amplification targets
Long prospective targets to ~ 300 bp mtDNA length identified in mtDNA Cambridge reference sequence revision using Primer Express software (Applied Biosystems) with a modified standard analysis settings. Primary and hydrolysis probe sequences to be generated three (3) goals wondered Mitomap database [1] to avoid common single nucleotide polymorphisms (SNPs), which, if present in the sample, it can reduce the binding to the template and therefore lead to inefficient amplification. Primer and probe are identified by Primer Express, some synthesized slumped to reduce the presence of a particular SNP, which is used in Advanced Master Mix (Applied Biosystems) rapid reaction strengthened in 7500 Real Time PCR System using Real Time PCR Software HID v1. 2 (Applied Biosystems) to collect and analyze data qPCR. qPCR reaction conditions and analysis software settings are optimized and modified to produce amplification efficient and powerful results.
QPCR experiments exported to Excel (Microsoft Corp.) for analysis and additional evaluation. The data used to develop the triplex qPCR methods, which include the amplification of the target long one, to measure and assess the degradation of human mtDNA, previously published results [2]. Triplex method that also included an internal positive control to test for the presence of amplification inhibitors in the sample [3]. The data presented here can be used to develop alternative amplification methods for biomedical applications specific users.
Autophagy supports cellular and organism homeostasis. However, whether autophagy should be inhibited or activated for cancer therapy remains unclear. Essential autophagy gene deletions increase the sensitivity of the mouse mammary carcinoma cells to radiation therapy in vitro and in vivo (in a syngeneic immunocompetent hosts). autophagy-deficient cells secreted increased amounts of type I interferon (IFN), which can be limited by CGAS or STING knockdown, depletion of mitochondrial DNA or mitochondrial outer membrane Permeabilisasi blockage via excess Bcl2 or BAX removal. In vivo, irradiated autophagy-competent mammary tumors induce strong immunity, which leads to an increase in lesion control nonirradiated away through type I IFN signaling systemic.
Developmental data for several human mitochondrial DNA (mtDNA) long amplification targets
Association of mitochondrial DNA copy number and rate of elimination with early pregnancy loss
early pregnancy loss (EPL) is a worldwide public events. Previous research suggests that mitochondrial DNA (mtDNA) copy number (CN) is associated with poor semen parameters and survival of preimplantation embryos, which shows the potential of mtDNA CN predictions for the results of an ongoing pregnancy.
However, no relevant studies have assessed the relationship between mtDNA CN and EPL. Thus, we aimed to determine whether mtDNA CN and 4977-bp mtDNA deletion rate (DR) in chorionic villi tissue associated with the EPL. Total DNA was extracted from the chorionic villi tissue of 75 cases of EPL and 75 healthy controls. chromosome analysis was performed using the copy number variation (CNV) sequencing. The mtDNA CN and DR is measured in a sample without pathogenic CNV. The relationship between mtDNA CN or DR and EPL risks were estimated using logistic regression.
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
Description: Cell Biolabs? Rapid Antibody Purification kit is designed for rapid, single-step purification of high-quality IgG from ascites, serum and tissue culture media or hybridoma supernatants.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
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Description: Ultracentrifugation of lipoproteins such as HDL, LDL and VLDL can be tedious and time consuming. The HDL Purification Kit provides comparable purity without the need for an ultracentrifuge. Samples are separated into fractions containing HDL and then further purified using standard table-top centrifugation speeds.
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.
Description: Ultracentrifugation of lipoproteins such as HDL, LDL and VLDL can be tedious and time consuming. The LDL/VLDL Purification Kit provides comparable purity without the need for an ultracentrifuge. Samples are separated into fractions containing LDL plus VLDL and then further purified using standard table-top centrifugation speeds.
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Human DNA topoisomerase I, mitochondrial (TOP1MT) ELISA Kit
Mitochondria of all organisms contain a genome that is distinct from that of the nucleus. The circular mitochondrial chromosome contains 37 genes that encode the RNA components of the mitochondrial translational apparatus, i.e., 22 transfer RNAs and
Description: Quantitative sandwich ELISA for measuring Human DNA-directed RNA polymerase, mitochondrial (POLRMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human DNA-directed RNA polymerase, mitochondrial (POLRMT)
Mitochondria of all organisms contain a genome that is distinct from that of the nucleus. The circular mitochondrial chromosome contains 37 genes that encode the RNA components of the mitochondrial translational apparatus, i.e., 22 transfer RNAs and
Description: Quantitative sandwich ELISA for measuring Human DNA-directed RNA polymerase, mitochondrial (POLRMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human DNA-directed RNA polymerase, mitochondrial (POLRMT)
Mitochondria of all organisms contain a genome that is distinct from that of the nucleus. The circular mitochondrial chromosome contains 37 genes that encode the RNA components of the mitochondrial translational apparatus, i.e., 22 transfer RNAs and
Description: Quantitative sandwich ELISA for measuring Human DNA-directed RNA polymerase, mitochondrial (POLRMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
EPL group had mtDNA CN significantly different (P <0.001) and DR (P = 0.005) compared with the control group. Both biomarkers are independent risk factors for EPL (CN odds ratio 1.71, 95% confidence interval 1.17 to 2.49, P = 0.005; DR odds ratio 1.07, 95% confidence interval 1.02 to 1.12 , P = 0.006). These results indicate that higher levels of mtDNA CN and DR strongly associated with the EPL and represent an independent risk factor for the EPL. Further studies validate these findings and to explore the biological mechanisms that underlie guaranteed.
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 Spin Antibody Purification Kit)
 Spin Antibody Purification Kit)
