Reduced mitochondrial DNA copy number is associated with the haplogroup, and some clinical features of breast cancer in Mexican patients
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Reduced mitochondrial DNA copy number is associated with the haplogroup, and some clinical features of breast cancer in Mexican patients
Mitochondrial DNA (mtDNA) copy number and mitochondrial DNA haplogroups have been associated with various types of cancer, including breast cancer, because they alter the cell’s energy metabolism. However, if the mtDNA copy number or haplogroup predictor of the risk is related to oxidative stress in human breast cancer tissue in Mexican patients remains to be determined. Using quantitative real-time PCR and sequencing of the region hypervariable mtDNA, analysis of mtDNA copy numbers in 82 breast cancer tissue (BCT) and matched adjacent tissues of normal (NAT) is performed to determine whether the number of copies correlate with clinical features and haplogroup Amerindian (A2 , B2, B4, C1 and D1).
The results showed that the mtDNA copy number decreased significantly in BCT compared with NAT (p = 0.010); it decreased significantly at BCT and NAT in women> 50 years, compared with NAT in women <50 years (p = 0.032 and p = 0.037, respectively); was significantly decreased in NAT and BCT at menopause and in the BCT group in the premenopausal group compared to NAT in the premenopausal group (p = 0.011, p = 0.010 and p = 0.018, respectively); and it also significantly decreased the group members BCT were classified as invasive ductal carcinoma I-III (IDC-I, IDC-II and IDC-III) and the IDC-II for NAT compared with IDC-I NAT (p = 0.025, p = 0.022 and p = 0.031 and p = 0.020, respectively).
MtDNA number of copies to BCT from patients with haplogroup B2 decreased compared with patients with D1 haplogroup (p = 0.01); for BCT patients with haplogroup C1 also declined compared with their counterparts NAT (p = 0.006) and with BCT patients belonged to haplogroup A2 and D1 (p = 0.01 and p = 0.03, respectively). In addition, the number of copies of mtDNA is the reduction of the order with three deletion relative to rCRS at nucleotide position A249del, A290del and A291del, or C16327T polymorphism with the same p = 0.019 for all four variants.
Conversely, increased copy number in the sequence containing C16111T, or T16362C G16319A polymorphism (p = 0.021, = 0.048, and 0.001, respectively). In conclusion, the decline in the number of copies of mtDNA in BCT compared to NAT indicated by the results, which showed an imbalance in oxidative phosphorylation (OXPHOS) which may affect the apoptotic pathway and the development of cancer. It was also observed an increase in the number of copies in the sample with certain polymorphisms, which may be a good sign of a favorable prognosis.
Reduced mitochondrial DNA copy number is associated with the haplogroup, and some clinical features of breast cancer in Mexican patients
Mitochondrial DNA Diversity in Large White pigs in Russia
The Great White pig is most often brought commercial pig breed in the world. The purpose of this study was to investigate the mtDNA D-loop in the White pig large (n = 402) from a variety of options raised in the Russian Federation from 2000 to 2019. The general sample consists of three groups: Old (n = 78) (Russian selection, 2000-2010); Imp (n = 123) (imported into Russia in 2008-2014); New (n = 201) (2015-2019). score (Fz) were synthesized calculated by analyzing the main PCA (principal components analysis).
Haplogroup affiliation to Asia or Europe is determined in accordance with the NCBI (National Center for Biotechnology Information). In the study, we defined 46 polymorphic sites and 42 haplotypes. significant differences between the groups Old, new Imp and haplotype and haplogroup frequencies established. Asian and European distribution haplotype in the group Old: 50% / 50%, Imp: 43% / 57%, New: 75% / 25%, respectively.
Rat DNA topoisomerase I, mitochondrial, TOP1MT ELISA Kit
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
*For IHC use only*
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
*For IHC use only*
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
*For IHC use only*
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
*For IHC use only*
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Various haplotype and haplogroup in pigs from New group associated with farm where they breed. haplotype frequencies were significantly different between groups Old_Center, Old_Siberia and Old_South. This study will provide information on the genetic diversity of Large White pig breed. The results will be useful for the conservation and sustainable use of these resources.
 ELISA Kit)
 ELISA Kit)
 ELISA Kit)
 I, Mitochondrial (TOP1MT))
)
 Antibody)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), 0.2mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), 1mg/mL)
)
 Antibody (HRP))
 Antibody (FITC))
 Antibody (Biotin))
 I, Mitochondrial (TOP1MT); Clone TOP1MT/488 (Concentrate))
 Protein)
 (TOP1MT/2883R) Antibody)
)
)
 (TOP1MT/2883R), 1mg/mL)
 (TOP1MT/2883R), 0.2mg/mL)
 Antibody)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), CF405S conjugate, 0.1mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), CF594 conjugate, 0.1mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), CF488A conjugate, 0.1mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), CF647 conjugate, 0.1mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), CF640R conjugate, 0.1mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), CF568 conjugate, 0.1mg/mL)
 I, Mitochondrial (TOP1MT)(TOP1MT/568), Biotin conjugate, 0.1mg/mL)
)
