Lifestyle and behavioral factors and mitochondrial DNA copy number in a diverse cohort of mid-life and older adults

Mitochondrial DNA copy number (mtDNAcn) is suspected of oxidative stress biomarkers and biological aging. modifiable factors, including physical activity (PA), avoiding heavy alcohol use and smoking, and maintaining good mental health, can reduce oxidative stress and improve healthy aging. However, limited data exists about how these factors relate to mtDNAcn or whether age, gender or race / ethnicity moderate association.

In a cross-sectional study, we chose 391 adults (183 non-Hispanic white, 110 black and 98 Hispanic; mean = 67 years) of the VITAL-DEP (vitamin D and omega-3 trial-Depression Endpoint Prevention) for a trial VITAL. We estimate the relationship between lifestyle and behavioral factors (PA, alcohol consumption, smoking and depression) and log-transformed mtDNAcn using multivariate linear regression model. MtDNAcn not correlate with chronological age; women have ~ 17% higher than men mtDNAcn.

There is no significant relationship between measures PA (frequency, amount or intensity) or consumption of alcohol by mtDNAcn. smoking (per 5 pack-years) was significantly associated with mtDNAcn (percent difference = -2.9% (95% confidence interval (CI) = -5.4%, -0.4%)); big contrast was observed between non-smokers versus severe (≥30 pack-years vs 0): The percent difference = -28.5% (95% CI = -44.2%, -8.3%). Estimates are suggestive of different mtDNAcn for the past vs no history of depression (difference in percent = -15.1% 95% CI = -30.8%, 4.1%), but this difference was not statistically significant.

The relationship between smoking and log-mtDNAcn varies by sex and race / ethnicity; it is stronger in males and Black participants. While the findings opportunity can not be ruled out, the results of this study support the association of smoking, but age is not chronological, with mtDNAcn and suggested a nuanced consideration mtDNAcn as an indication of the different countries oxidative stress vs. biological aging itself.

 Lifestyle and behavioral factors and mitochondrial DNA copy number in a diverse cohort of mid-life and older adults
Lifestyle and behavioral factors and mitochondrial DNA copy number in a diverse cohort of mid-life and older adults

and assembled the complete mitochondrial genome DNA analysis of Lutjanus Fulgens barcode (Valenciennes, 1830) and a comparison with other species Lutjanus

Lutjanus Fulgens (Valenciennes, 1830) is a teleost species classified in the family Lutjanidae which is the original of the Eastern Atlantic Ocean. Although very commercialized because of the abundance and good taste, the production output has declined in recent years.

This is an indication of the need for measures of effective management and conservation. However, an accurate species identification will ensure strategic management and conservation measures. DNA-based species identification has proven its reliability in this case through the proper species identification. Some researchers have confirmed the accuracy of DNAbarcode as a means of identification of species as well as species phylogeny analysis based on both mitogenome complete and COI gene.

Currently, nine specimens of L. Fulgens that samples from Ghana and the target DNA-based analysis, namely, the complete mitochondrial COI gene DNAand (barcode DNA) analysis. The results revealed that L. Fulgens mitogenomic consisted of 16,500 base pairs (bp) mtDNA consisting of 22 RNA transfer 13 protein-coding genes, two ribosomal RNA (GenBank Accession Number: MN398650). In addition, sequence analysis of polymorphisms COIgene (MN986442-MN986450) detected two haplotypes.

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 686
Description: Isolates mt. DNA, no genomic DNA contamination

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Mitochondrial DNA Isolation Kit, Catalog: PI-0105

PI-0105 50 isolations
EUR 465
  • N/A
Description: To isolate intact mitochondrial DNA from a variety of cells and tissue types without contamination from genomic DNA. 

Mitochondrial Protein Isolation Buffer

22050010-1 30 mL
EUR 76.34

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Cell Mitochondria Isolation Kit

ETF3601 100 assays
EUR 135

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6
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Cell Mitochondria Isolation Kit

abx090630-100l 100 µl
EUR 187.5

Cell Mitochondria Isolation Kit

abx090630-1ml 1 ml Ask for price

Cell Mitochondria Isolation Kit

abx090630-200l 200 µl Ask for price

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

Tissue Mitochondria Isolation Kit

ETF3606 100 assays
EUR 135

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6
  • Shipped within 5-10 working days.

Tissue Mitochondria Isolation Kit

abx090631-100l 100 µl
EUR 187.5

Tissue Mitochondria Isolation Kit

abx090631-1ml 1 ml Ask for price

Tissue Mitochondria Isolation Kit

abx090631-200l 200 µl Ask for price

Mitochondria Isolation Kit (Cultured Cells)

K1138-10 10rxns
EUR 28
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-20 20rxns
EUR 44
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-30 30rxns
EUR 56
Description: Mitochondria Isolation Kit

Mammalian Mitochondria Isolation Kit for Tissue

abx098857-50rxns 50 rxns
EUR 493.2
  • Shipped within 5-10 working days.

Mammalian Mitochondria Isolation Kit for Tissue

abx098857-100l 100 µl
EUR 912.5

Mammalian Mitochondria Isolation Kit for Tissue

abx098857-1ml 1 ml Ask for price

Mammalian Mitochondria Isolation Kit for Tissue

abx098857-200l 200 µl Ask for price

Mitochondria Isolation Kit (Mammalian cells / tissues)

P507L NULL Ask for price

Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)

MP-007 each
EUR 535

Mammalian Mitochondria Isolation Kit for Cultured Cells

20-abx098856
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  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Mammalian Mitochondria Isolation Kit for Cultured Cells

abx098856-100l 100 µl
EUR 912.5

Mammalian Mitochondria Isolation Kit for Cultured Cells

abx098856-1ml 1 ml Ask for price

Mammalian Mitochondria Isolation Kit for Cultured Cells

abx098856-200l 200 µl Ask for price

Mitochondria Isolation Kit for Tissue and Cultured Cells

KC010100 1 kit
EUR 357

Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests)

MM-038 each
EUR 535

Mammalian Mitochondria Isolation Kit For Tissue & Cultured Cells

K288-50 each
EUR 502.8

Urine DNA Isolation Kit

K5011150 1 kit
EUR 261

Stool DNA Isolation Kit

K5011250 1 kit
EUR 261

Serum DNA Isolation Kit

K5018100 1 kit
EUR 391

Saliva DNA Isolation Kit

AG0226 Kit Ask for price
Description: Saliva DNA Isolation Kit provides a fast and simple spin column procedure for isolating high quality DNA from saliva samples collected and preserved using AcceGen's Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using AcceGen's kit is of the excellent quality, and is compatible with a number of downstream research applications including PCR, qPCR, Southern Blot analysis, sequencing and microarray analysis.

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 261

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 273
  • Assay Kit containing the folllowing: Cell Lysis Buffer, Enzyme Mix (lyophilized), TE Buffer
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

GWB-AXR187 50 assays Ask for price

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 389
Description: Isolates highly pure Genomic DNA within 90 min.

Genomic DNA Isolation Kit

abx460025-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-96tests 96 tests
EUR 437.5

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

FitAmp Urine DNA Isolation Kit 

P-1017
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  • 100 Samples
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  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

Genomic DNA Isolation Kit (Plant)

PDC10-0100 100 rxns
EUR 132

Viral DNA/RNA Isolation Kit

AGDK-0044 Kit Ask for price
Description: The Viral DNA/RNA Isolation Kit is intended for rapid co-extraction of viral DNA and RNA from a variety of biofluid samples, such as, plasma, serum, milk and swap samples. The proprietary microspherical paramagnetic beads used in the kit have a large binding surfaces and a high affinity towards nucleic acids. Going through sample lysis/binding, washing, and elution steps, the whole process can be completed under 35 minutes, and yields highly pure nucleic acids elute.  The recovered nucleic acids can be used in a wide range of applications, such as PCR, RT-PCR, Sanger Sequencing, NGS, and gene chips.

Human mitochondrial RNA polymerase - for 100 assays

MRPA-100 100 assays
EUR 426.25
Description: This product includes 33 ul of 7.5 uM Human Mitochondrial RNA Polymerase

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

Genomic DNA Isolation Kit (Tissue)

PDC11-0100 100 rxns
EUR 132

Magnetic Cell-Free DNA Isolation Kit, 50 reactions/pk

208301 6 pcs/cs
EUR 183.37
Description: Magnetic Cell-Free DNA Isolation Kit, 50 reactions/pk

JC-1 Mitochondrial Membrane Potential Detection Kit (100 assays)

30001-1 KT
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Magnetic Blood Genomic DNA Isolation Kit, 50 reactions/pk

208501 8 pcs/cs
EUR 74.91
Description: Magnetic Blood Genomic DNA Isolation Kit, 50 reactions/pk

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

JC-1 Mitochondrial Membrane Potential Detection Kit (25 assays)

30001-T 1kit
EUR 87
  • Cytology test kits or supplies
Description: N/A

JC-1 Mitochondrial Membrane Potential Detection Kit (25 assays)

30001-T-1 KT
EUR 87

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 391

Genomic DNA isolation dual kit

PDC02-0100 100 rxns
EUR 110

HipurA® Cotton DNA Isolation Kit

MB528-250PR 1 unit
EUR 213.83
Description: HipurA® Cotton DNA Isolation Kit

HipurA® Cotton DNA Isolation Kit

MB528-50PR 1 unit
EUR 47.85
Description: HipurA® Cotton DNA Isolation Kit

HiPurA® Banana DNA Isolation Kit

MB533-250PR 1 unit
EUR 182.59
Description: HiPurA® Banana DNA Isolation Kit

HiPurA® Banana DNA Isolation Kit

MB533-50PR 1 unit
EUR 46.63
Description: HiPurA® Banana DNA Isolation Kit

Geneaid™ DNA Isolation Kit (Blood)

GEB003 each
EUR 3

Geneaid™ DNA Isolation Kit (Blood)

GEB01K each
EUR 450

Geneaid™ DNA Isolation Kit (Blood)

GEB100 each
EUR 75

Geneaid™ DNA Isolation Kit (Yeast)

GEY004 each
EUR 3

Geneaid™ DNA Isolation Kit (Yeast)

GEY100 each
EUR 64.5

Geneaid™ DNA Isolation Kit (Yeast)

GEY300 each
EUR 174

Apoptotic DNA Ladder Isolation Kit

GWB-AXR127 50 assays Ask for price

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 490
Description: No genomic DNA contamination.

Geneaid™ DNA Isolation Kit (Tissue)

GET005 each
EUR 3

Geneaid™ DNA Isolation Kit (Tissue)

GET1.5K each
EUR 550

Geneaid™ DNA Isolation Kit (Tissue)

GET150 each
EUR 92.5

Dual Genomic DNA Isolation Kit (Plant)

PDC05-0100 100 rxns
EUR 154

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
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  • 100 Samples
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  • 100 Samples

Geneaid™ DNA Isolation Kit (Bacteria)

GEE005 each
EUR 3

Geneaid™ DNA Isolation Kit (Bacteria)

GEE1.5K each
EUR 450

Geneaid™ DNA Isolation Kit (Bacteria)

GEE150 each
EUR 75

Dual Genomic DNA Isolation Kit(Tissue)

PDC06-0100 100 rxns
EUR 154

Genomic DNA Isolation Reagent Kit

abx460021-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Reagent Kit

abx460021-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Reagent Kit

abx460021-96tests 96 tests
EUR 325

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 91.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Fungi

Plant Tissue Genomic DNA Isolation Kit

K316-100 each
EUR 451.2

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Magnetic Blood Spots Genomic DNA Isolation Kit, 50 reactions/pk

208401 8 pcs/cs
EUR 74.91
Description: Magnetic Blood Spots Genomic DNA Isolation Kit, 50 reactions/pk

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 91.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

Mammalian Cell Genomic DNA Isolation Kit

K967-100 each
EUR 457.2

HiPurA® SPP Blood DNA Isolation Kit

MB541-10ML 1 unit
EUR 27.19
Description: HiPurA® SPP Blood DNA Isolation Kit

HiPurA® SPP Blood DNA Isolation Kit

MB541-150ML 1 unit
EUR 155.06
Description: HiPurA® SPP Blood DNA Isolation Kit

HiPurA® SPP Blood DNA Isolation Kit

MB541-300ML 1 unit
EUR 242.51
Description: HiPurA® SPP Blood DNA Isolation Kit

HiPurA® SPP Blood DNA Isolation Kit

MB541-50ML 1 unit
EUR 68.71
Description: HiPurA® SPP Blood DNA Isolation Kit

Blood RNA Isolation(50) Kit     

TBS6002 50 samples
EUR 198

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 241.2

Soil DNA Isolation Mini Kit (100prep)

FASOI_001-1 1 kit
EUR 90

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 189.6

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 259.2

Soil DNA Isolation Mini Kit (50prep)

FASOI_001 1 kit
EUR 45

Geneaid™ DNA Isolation Kit (Blood) Plus

GEB01K-A each
EUR 480

Geneaid™ DNA Isolation Kit (Tissue) Plus

GET1.5K-A each
EUR 594

FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
EUR 241.2

Geneaid™ DNA Isolation Kit (Cultured Cell)

GEC005 each
EUR 3

Geneaid™ DNA Isolation Kit (Cultured Cell)

GEC1.5K each
EUR 360

Geneaid™ DNA Isolation Kit (Cultured Cell)

GEC150 each
EUR 60

Geneaid™ DNA Isolation Kit (Bacteria) Plus

GEE1.5K-A each
EUR 480

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 192

FastPure Plant DNA Isolation Mini Kit

DC104-01 50 rxns
EUR 60

Genomic DNA Isolation Reagent

PDR01-0100 100 rxns
EUR 88

HiPurA® Plant DNA Isolation Kit (CTAB Me

MB502-50PR 1 unit
EUR 33.57
Description: HiPurA® Plant DNA Isolation Kit (CTAB Me

Plant Genomic DNA Isolation Reagent Kit

abx460022-1096tests 10 × 96 tests Ask for price

Plant Genomic DNA Isolation Reagent Kit

abx460022-596tests 5 × 96 tests Ask for price

Plant Genomic DNA Isolation Reagent Kit

abx460022-96tests 96 tests
EUR 325

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
EUR 94.72
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
EUR 198.85
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 212.4

FastPure Bacteria DNA Isolation Mini Kit

DC103-01 100 rxns
EUR 52

Column-Pure Animal Genomic DNA Isolation Kit

D512 50 Preparations
EUR 195

FitAmp General Tissue Section DNA Isolation Kit 

P-1003
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  • 100 Samples
  • 50 Samples
  • 100 Samples

HipurA® Silica Kit for DNA Isolation

MB503-400PR 1 unit
EUR 47.85
Description: HipurA® Silica Kit for DNA Isolation

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
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  • 100 Samples
  • 50 Samples
  • 100 Samples

Column-Pure Bacterial Genomic DNA Isolation Kit

D511 50 Preparations
EUR 195

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064
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  • 50 Isolations
  • 25 Isolations
  • 50 Isolations

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
EUR 214.8

FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
EUR 472.8

Geneaid™ DNA Isolation Kit (Cultured Cell) Plus

GEC1.5K-A each
EUR 400

Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

208601 8 pcs/cs
EUR 131.12
Description: Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

Genomic DNA Isolation Kit (Paraffin-embedded tissue)

PDC12-0100 100 rxns
EUR 132

Soil DNA Plus Isolation Mini Kit (100prep)

FAPSO-001-1 100 preps
EUR 241.2

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
EUR 212.4

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102-01 100 rxns
EUR 52

Plasmid DNA Isolation Solution I

10760028-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution I

10760028-2 500 mL
EUR 45.3

Plasmid DNA Isolation Solution II

10760029-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution II

10760029-2 500 mL
EUR 37.89

Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
EUR 189.6

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

Plasmid DNA Isolation Solution III

10760030-1 250 mL
EUR 28.07

Plasmid DNA Isolation Solution III

10760030-2 500 mL
EUR 43

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC09-0100 100 rxns
EUR 99

Plant Genomic DNA Isolation Reagent

PDR02-0100 100 rxns
EUR 88

Mitochondria/Cytosol Fractionation Kit - 100 assays

GWB-AXR169 100 assays Ask for price

Mitochondria/Cytosol Fractionation Kit - 25 assays

GWB-AXR170 25 assays Ask for price

QC Buffer for Plasmid DNA Isolation

10760036-1 250 mL
EUR 25.45

QC Buffer for Plasmid DNA Isolation

10760036-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation

10760037-1 250 mL
EUR 24.44

QF Buffer for Plasmid DNA Isolation

10760037-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation

10760037-3 1 L
EUR 76.87

One Tube Viral DNA Isolation Buffer

VT4785 50Preps
EUR 224.44
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

One Tube Viral DNA Isolation Buffer

VT4786 100preps
EUR 388.86
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

This haplotype both collected from the same fish landing site which suggests the possibility of cryptic diversity in the population linage L. Fulgens in Vodza. According to the examination phylogeny, taxonomy close relationship exists between L. Fulgens and Lutjanus buccanella caused by the recent evolution called sympatric speciation. This study serves as a new study for this species, building the foundation for the study of molecule-based future for this species and as reference DNA barcode data.